zombie aqua fixable viability dye (Thermo Fisher)

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Thermo Fisher zombie aqua fixable viability dye
Zombie Aqua Fixable Viability Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zombie+aqua+fixable+viability+dye/zombie+aqua+fixable+viability+dye/pm39443808-467-9-14
Average 90 stars, based on 1 article reviews

zombie aqua fixable viability dye - by Bioz Stars, 2026-10

90/100 stars

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Article Title: Dynamic CD8+ T cell responses to cancer immunotherapy in human regional lymph nodes are disrupted by metastasis
Article Snippet: .. Cells were then incubated with Human TruStain FcX (Biolegend) to prevent non-specific antibody binding before staining with Zombie Aqua Fixable Viability Dye (Thermo Fisher) and anti-human CD45 antibody (Thermo Fisher) in PBS/2%FCS/2mM EDTA/0.01% sodium azide and incubated for 25 minutes on ice in the dark. .. Live CD45+ and CD45-cells were sorted on a BD FACSAria Fusion.

Article Title: Proinflammatory immune cells disrupt angiogenesis and promote germinal matrix hemorrhage in prenatal human brain
Article Snippet: The cells were then stained with acridine orange/propidium iodide (AO/PI) cell viability dye and counted with the Cellaca MX High-throughput Automated Cell Counter (Nexelcom). .. Approximately 3 million cells were aliquoted from each sample into a 96-v-well plate (Corning) and incubated with Zombie Aqua Fixable Viability Dye (Thermo) for 20 min on ice and in the dark. .. After viability dye incubation, the cells were washed with sort buffer (PBS/2% FCS/2 mM EDTA) and incubated with Human Fcx (BioLegend) to block non-specific antibody binding.

Article Title: Chromatin remodelling drives immune cell-fibroblast communication in heart failure.
Article Snippet: Michael Alexanian1,2,3,21 ✉, Arun Padmanabhan, Tomohiro Nishino, Joshua G. Travers, Lin Ye, Angelo Pelonero, Clara Youngna Lee, Nandhini Sadagopan, Yu Huang, Kirsten Auclair, Ada Zhu, Yuqian An, Christina A. Ekstrand, Cassandra Martinez, Barbara Gonzalez Teran, Will R. Flanigan, Charis Kee-Seon Kim, Koya Lumbao-Conradson, Zachary Gardner, Li Li, Mauro W. Costa, Rajan Jain, Israel Charo, Alexis J. Combes, Saptarsi M. Haldar, Katherine S. Pollard, Ronald J. Vagnozzi, Timothy A. McKinsey, Pawel F. Przytycki & Deepak Srivastava

Article Title: MK256 is a novel CDK8 inhibitor with potent antitumor activity in AML through downregulation of the STAT pathway.
Article Snippet: .. Cells were subsequently incubated with Zombie Aqua Fixable Viability Dye (Thermo), washed with Sort buffer, and incubated with Human FcX (BioLegend) to prevent nonspecific antibody binding. .. Following FcX incubation, cells were washed with Sort buffer and incubated with cell surface antibody mix diluted in the BV stain buffer (BD Biosciences) following manufacturer instructions for 30 mins on ice in the dark.

Article Title: Proinflammatory immune cells disrupt angiogenesis and promote germinal matrix hemorrhage in prenatal human brain.
Article Snippet: Cells were then stained with AO/PI cell viability dye and counted with the Cellaca MX High-throughput Automated Cell Counter (Nexelcom). .. Approximately 3 million cells were aliquoted from each sample into a 96 v-well plate (Corning), and incubated with Zombie Aqua Fixable Viability Dye (Thermo) for 20 minutes on ice and in the dark. .. After viability dye incubation, cells were washed with sort buffer (PBS/2% FCS/2mM EDTA) and incubated with Human Fcx (Biolegend) to block non-specific antibody binding.

Article Title: MK256 is a novel CDK8 inhibitor with potent antitumor activity in AML through downregulation of the STAT pathway
Article Snippet: .. Cells were subsequently incubated with Zombie Aqua Fixable Viability Dye (Thermo), washed with Sort buffer, and incubated with Human FcX (BioLegend) to prevent non-specific antibody binding. .. Following FcX incubation, cells were washed with Sort buffer and incubated with cell surface antibody mix diluted in the BV stain buffer (BD Biosciences) following manufacturer instructions for 30 mins on ice in the dark.

Article Title: Proinflammatory immune cells disrupt angiogenesis and promote germinal matrix hemorrhage in prenatal human brain.
Article Snippet: The cells were then stained with acridine orange/propidium iodide (AO/PI) cell viability dye and counted with the Cellaca MX Highthroughput Automated Cell Counter (Nexelcom). .. Approximately 3 million cells were aliquoted from each sample into a 96vwell plate (Corning) and incubated with Zombie Aqua Fixable Viability Dye (Thermo) for 20 min on ice and in the dark. .. After viabil ity dye incubation, the cells were washed with sort buffer (PBS/2% FCS/2 mM EDTA) and incubated with Human Fcx (BioLegend) to block nonspecific antibody binding.

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Staining:

Article Title: Discovering dominant tumor immune archetypes in a pan-cancer census.
Article Snippet: Cells were then washed in DPBS and incubated with Zombie Aqua Fixable Viability Dye (Thermo). .. Following viability dye, cells were washed with sort buffer and incubatedwith cell surface antibodiesmix diluted in theBV stain buffer (BDBiosciences) followingmanufacturer instruction for 30minutes on ice in the dark and subsequently fixed in either Fixation Buffer (BD Biosciences) or in Foxp3/Transcription Factor Staining Buffer Set (eBioscience) if intracellular staining was required. .. Following viability dye, cells were washed with sort buffer and incubatedwith cell surface antibodiesmix diluted in theBV stain buffer (BDBiosciences) followingmanufacturer instruction for 30minutes on ice in the dark and subsequently fixed in either Fixation Buffer (BD Biosciences) or in Foxp3/Transcription Factor Staining Buffer Set (eBioscience) if intracellular staining was required.

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